Benchmark Scientific PR4100-500
Accuris SmartCheck 1kb DNA Ladder, 5 x 500ul/500 lanes, 0.1 mg/ml Concentration
PR4100500
PR4100-500
Benchmark Scientific PR4100-500
Accuris SmartCheck 1kb DNA Ladder, 5 x 500ul/500 lanes, 0.1 mg/ml Concentration
PR4100500
PR4100-500
The Benchmark Scientific PR4100500 SmartCheck 1kb DNA Ladder is designed to determine the size of DNA fragments in most standard lab applications. The ready-to-use formulation includes both a loading buffer and tracking dye. The ultra pure production allows for economical ambient shipping and the ladder itself features higher intensity reference bands.
- Size Range: 5 x 500 µl
- Number of Lanes: 500
- Concentration: 0.1 mg/mL
- 1 kb Size range
- Includes 1 DNA Ladders tubes
- Ready-to-use formulation includes loading buffer and tracking dye
- 500 ul suitable for 100 lanes (5 ul per lane)
- Higher intensity reference bands
- Ultra pure production allow economical ambient shipping
- Technical Details
- Protocol: Briefly vortex the tube and use 5 ul per lane. Additional loading buffer is not required
- Concentration: 0.1 mg/ml
- Tracking dye: bromophenol blue and xylene cyanol
- Buffer formulation: 10 mM Tris-HCI (pH 8.0), 5 mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol
- Shipping and Storage: SmartCheck DNA Ladders are shipped at ambient temperature. On arrival, store at -20 C for optimum stability and long-term storage up to 15 months.
- Production: SmartCheck ladders are produced from proprietary plasmids, digested to completion. A multistep chromatography method is used to ensure purity and DNA quality.
- Quality Control: Each lot of SmartCheck DNA Ladders is tested with a spectrophotometer to confirm DNA concentration, as well as agarose electrophoresis to confirm band sharpness.
The Benchmark Scientific PR4100500 Accuris SmartCheck 1kb DNA Ladder 5-pack is produced from proprietary plasmids, digested to completion and purified in a multi-step chromatography process. The ladders are supplied in a ready-to-use format that includes loading buffer and tracking dyes. The ladders are supplied at a concentration of 0.1 mg/ml, in a buffer consisting of 10mM Tris-HCl (pH 8.0), 5mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol.
Storage
This product is shipped at ambient temperature. Upon receipt, immediately store at -20° C. Ladders can be stored for up to 15 months at -20° C or at 4° C for up to 6 months.
Quality Control
The ladders are purified by a multi-step chromatography method. Band sharpness is verified by agarose electrophoresis and DNA concentration is verified spectrophotometrically.
General Guidelines
PR4100-500 Accuris SmartCheck DNA ladders are supplied ready-to-use and comes with 5 tubes of 500ul, for up to 500 lanes. Briefly vortex the tube and add 5μl to the desired well in the gel. If the ladder is frozen, allow time to thaw and mix thoroughly. The SmartCheck Ladder can be used to approximate the mass of DNA in samples of similar size by comparing the intensity of the sample band to that of the ladder. The masses of each DNA band per 5μl load are given on the back side of this insert.
| Brand | Benchmark Scientific |
|---|---|
| Condition | NEW |
| Vendor Alias | PR4100-500 |
- 5 - DNA tubes with loading dye
