The Benchmark Scientific MR9620-E SmartReader MultiMode is a multi-detection instrument capable of performing absorbance, fluorescence, and luminescence-based readings. Equipped with a 10-inch color touch screen, programming and operation is performed on-board. The built-in software allows for multiple algorithmic analyses of standard curves, qualitative and quantitative measurements, kinetics, spectroscopy, and more. An optional FDA 21 CFR Part 11 compliant security software (not included) is available for this instrument that allows for remote programming/operation via a PC. The MR9620-E unit comes equipped with a plug for 230V UK/EU/AU outlets.

The SmartReader MultiMode quickly processes assays in standard 96 well plates and can also accept 6, 12, 24, 48 and 384 well plates. Programming and operation are managed from the instrument's large color touch screen and no external computer or software is required. Optional PC software (not included) is available for easy control and data handling via a Windows PC. The friendly and intuitive interface guides the user through various options for assay set up and processing. The instrument features a built-in plate shaker with linear, orbital, and double-orbital shaking modes, and an incubator that can perform top heating from ambient +4°C to 45°C. Top heating creates a temperature gradient to prevent condensation when lids are used on the microplates. Upon startup, the SmartReader MultiMode performs a self-diagnostic test which is followed by an automatic calibration of the entire system for ensuring consistent results across a wide range of assays.

UV-Visible Absorbance

Utilizing an advanced monochromator and a long-life, broad-spectrum xenon flash lamp, the SmartReader MultiMode can produce incident light at user-selected wavelengths from 200 to 1000 nanometers in 1 nanometer steps. Processing assays is fast in which a full 96 well plate can be read in under 15 seconds. The kinetic assay feature performs multiple readings over a set period of time with curves for each well displayed in real-time. The optional SmartDrop plate (not included) allows for fast and convenient UV absorbance evaluation of up to 16 nucleic acid and protein sample droplets. Some of the absorbance applications performed includes ELISA Assays, Colorimetrics Assays (Bradford, Lowry, etc.), Enzyme Kinetics, Spectral Scanning, DNA and RNA Analysis, and more.

Fluorescence

Three filter modules are included to provide the most common excitation and emission wavelengths needed for fluorescence assays. The first module includes an excitation wavelength of 470 nanometers and emission wavelength of 525 nanometers. The second module includes an excitation wavelength of 523 nanometers and emission wavelength of 564 nanometers. The third module includes an excitation wavelength of 624 nanometers and emission wavelength of 692 nanometers.

Additional filter modules (not included) are available. These advanced modules incorporate dichroic mirrors with narrow band-pass optical filters and ensure high light transmission, increased detection sensitivity, and are easily exchanged. Modules are barcoded for automatic loading of wavelength data. The SmartReader MultiMode allows for automatic dynamic range selection which allows for the gain of the photomultiplier tube to accurately capture and normalize the signal intensity of samples.

Luminescence

The SmartReader MultiMode is capable of luminescent detection and can process both flash and glow assays. An advanced PMT (photo multiplier tube) enhances sensitivity of weak signals, prevents over-saturation of strong signals, and provides an ideal detection range for all common luminescence applications. Up to 2 sample injectors (not included) can be installed and are compatible with both 96 and 384-well plates.

Data Transfer and PC Software

Data from the SmartReader MultiMode to can be exported as either a .XLS or .CSV file via the included USB flash drive, the FTP server, or by using the optional SmartReader PC Software (not included).

The SmartReader PC Software allows for remote programming and operation of the instrument via cable connection. Data from the instrument is automatically uploaded to the connected PC for further analysis. Both a Research version for academic use and a FDA 21 CFR Part 11 compliant version are available.